Enzymes and Inhibitors
New England Biolabs, Inc. Uracil-DNA Glycosylase (UDG) – 5000 units
E. coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).
New England Biolabs, Inc. β1-3 Galactosidase - 500 units
b1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of 1-3 and, at a much lower rate, 1-6 linked galactose residues from oligosaccharides.
New England Biolabs, Inc. CpG Methyltransferase (M.SssI) – 500 units
The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3'
New England Biolabs, Inc. T7 Exonuclease – 5000 units
T7 Exonuclease acts in the 5' to 3' direction, catalyzing the removal of 5' mononucleotides from linear or nicked duplex DNA.
New England Biolabs, Inc. T4 Polynucleotide Kinase Reaction Buffer – 4 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. ShortCut RNase III – 200 units
ShortCut RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18-25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1µ g of dsRNA into siRNA suitable for RNA interference in mammalian cells.
Active Motif Preindex Tn5 Transposomes 1x96
Pre-indexed Assembled Tn5 Transposomes are provided in a flexible breakaway 96-well plate format to enable higher throughput ATAC-Seq assays. Each well contains a transposome loaded with a unique i5 and i7 index, enabling direct indexing during tagmentation. By having a unique i7 & i5 Indexed Primer combination in each well, it is easy to perform multiple ATAC-Seq reactions without having to pipette primers separately and keep track of the combinations.
LGC GENOMICS LLC (Lucigen) Rec J Exonuclease, 250 U @ 10 U/LMagnesium-dependent exonuclease for 5' to 3' digestion of single-stranded DNA.
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 53 direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65C for 20 minutes.
Enzo Life Sciences Bio 17 ATP (250 nmol)
Alternative Name: Biotin-17-adenosine-5'-triphosphate. Formulation: Liquid. Solution in water. Purity: ≥93% (HPLC). Appearance: Clear, colorless liquid. Shipping: Shipped on Dry Ice. Long Term Storage: -20°C. Handling: Avoid freeze/thaw cycles.
LGC GENOMICS LLC (Lucigen) EpiScript RNase H- Reverse Transcriptase, 25,000 units @ 200 U/LAn optimised reverse transcriptase system for the production of full-length cDNA.
EpiScript™ Reverse Transcriptase an alternative to SuperScript II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activityProduce full-length cDNA with RNAse H-mutantAchieve higher specificity at elevated temperatures, up to 55 CKit Contents: EpiScript RNase H- Reverse Transcriptase, 10X Reaction Buffer, 100 mM DTT.
New England Biolabs, Inc. Mung Bean Nuclease – 7500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.
Carnabio Usa Inc BTN - LCK / 10UG
BTN-LCK, Full-length, Wild type, Amino Acid 1-509(end), NP_005347.2, 62 kDa, Insect (sf21); 10microg
Enzo Life Sciences HDAC1 (human), (recombinant) (His-tag) (50 µg)
Alternative name: Histone deacetylase 1. Formulation: Liquid. In 50mM TRIS, pH 8.0, 138mM sodium chloride and 10% glycerol. Source: Produced in insect cells. HDAC1 from human cDNA (482 aa). Produced in a baculovirus expression system. Long Term Storage: -80°C.
LGC GENOMICS LLC (Lucigen) CircLigase II ssDNA Ligase, 1,000 U @ 100 U/LAn ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i.e. circularisation) of ssDNA and ssRNA templates.
CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group
New England Biolabs, Inc. EcoRI Methyltransferase – 10000 units
EcoRI Methyltransferase modifies the internal adenine residue (N6) of the sequence GAATTC.